Molecular repertoire of Deinococcus radiodurans after 1 year of
Por um escritor misterioso
Last updated 20 setembro 2024
Background The extraordinarily resistant bacterium Deinococcus radiodurans withstands harsh environmental conditions present in outer space. Deinococcus radiodurans was exposed for 1 year outside the International Space Station within Tanpopo orbital mission to investigate microbial survival and space travel. In addition, a ground-based simulation experiment with conditions, mirroring those from low Earth orbit, was performed. Methods We monitored Deinococcus radiodurans cells during early stage of recovery after low Earth orbit exposure using electron microscopy tools. Furthermore, proteomic, transcriptomic and metabolomic analyses were performed to identify molecular mechanisms responsible for the survival of Deinococcus radiodurans in low Earth orbit. Results D. radiodurans cells exposed to low Earth orbit conditions do not exhibit any morphological damage. However, an accumulation of numerous outer-membrane-associated vesicles was observed. On levels of proteins and transcripts, a multi-faceted response was detected to alleviate cell stress. The UvrABC endonuclease excision repair mechanism was triggered to cope with DNA damage. Defense against reactive oxygen species is mirrored by the increased abundance of catalases and is accompanied by the increased abundance of putrescine, which works as reactive oxygen species scavenging molecule. In addition, several proteins and mRNAs, responsible for regulatory and transporting functions showed increased abundances. The decrease in primary metabolites indicates alternations in the energy status, which is needed to repair damaged molecules. Conclusion Low Earth orbit induced molecular rearrangements trigger multiple components of metabolic stress response and regulatory networks in exposed microbial cells. Presented results show that the non-sporulating bacterium Deinococcus radiodurans survived long-term low Earth orbit exposure if wavelength below 200 nm are not present, which mirrors the UV spectrum of Mars, where CO2 effectively provides a shield below 190 nm. These results should be considered in the context of planetary protection concerns and the development of new sterilization techniques for future space missions. Video Abstract
The scientific revolution that unraveled the astonishing DNA repair capacity of the Deinococcaceae: 40 years on
RCSB PDB - 2C2Q: The crystal structure of mismatch specific uracil-DNA glycosylase (MUG) from Deinococcus radiodurans. Inactive mutant Asp93Ala.
Molecular repertoire of Deinococcus radiodurans after 1 year of exposure outside the International Space Station within the Tanpopo mission, Microbiome
Life, Free Full-Text
Life, Free Full-Text
Life, Free Full-Text
Molecular response of Deinococcus radiodurans to simulated microgravity explored by proteometabolomic approach
Molecular repertoire of Deinococcus radiodurans after 1 year of exposure outside the International Space Station within the Tanpopo mission, Microbiome
The scientific revolution that unraveled the astonishing DNA repair capacity of the Deinococcaceae: 40 years on
A multidomain connector links the outer membrane and cell wall in phylogenetically deep-branching bacteria
A new perspective on radiation resistance based on Deinococcus radiodurans
PDF] Proteometabolomic response of Deinococcus radiodurans exposed to UVC and vacuum conditions: Initial studies prior to the Tanpopo space mission
Genes, Free Full-Text
The radioresistant and survival mechanisms of Deinococcus radiodurans
Memory Effect on the Survival of Deinococcus radiodurans after Exposure in Near Space
Recomendado para você
-
Deinococcus - microbewiki20 setembro 2024
-
Deinococcus Radiodurans: The World's Toughest Bacterium. A Review20 setembro 2024
-
Deinococcus_radiodurans-Lewis_Dartnell - Lewis Dartnell20 setembro 2024
-
Elucidation Of The Structure Of The Surface Layer Of Deinococcus20 setembro 2024
-
Deinococcus radiodurans by Angel G20 setembro 2024
-
Accumulation of Mn(II) in Deinococcus radiodurans Facilitates20 setembro 2024
-
Deinococcus radiodurans20 setembro 2024
-
Home - Deinococcus geothermalis DSM 1130020 setembro 2024
-
Lord of the Ring - Chemistry Weizmann Wonder Wander - News20 setembro 2024
-
How to pronounce Deinococcus radiodurans20 setembro 2024
você pode gostar
-
O Metaverso das Coisas: um novo mundo virtual20 setembro 2024
-
Nike Dri Fit Houston TX Pappas Burger Black Jacket Sz S Very Nice20 setembro 2024
-
Kengun Online Codes (April 2023) - Roblox20 setembro 2024
-
2110841 - safe, artist:soul-yagami64, character:fluttershy, character:rainbow dash, blushing, crossover, crossover shipping, female, kiss on the cheek, kissing, male, shadow the hedgehog, shipping, sonic the hedgehog, sonic the hedgehog (series20 setembro 2024
-
Champions League: Fiorentina 2-2 Slavia Prague20 setembro 2024
-
Pokémon UNITE: Gardevoir chega em julho; veja trailer20 setembro 2024
-
Zuma Lounge - Picture of Zuma Dubai - Tripadvisor20 setembro 2024
-
Koi to Producer - Evol x Love AMV20 setembro 2024
-
Cat Black, Free Stock Photo, Illustrated silhouette of a black cat20 setembro 2024
-
Chainsaw Man 2 - Bandas Desenhadas20 setembro 2024